Test Detail

Charcot-Marie-Tooth 4B2 demyelinating neuropathy (SBF2) — Miniature Schnauzer

Neurological · Dog

Hereditary demyelinating peripheral neuropathy of the Miniature Schnauzer, the canine equivalent of human Charcot-Marie-Tooth type 4B2. It presents with motor and sensory demyelination, abnormally folded myelin and characteristic signs of lower cranial nerve dysfunction (regurgitation due to megaesophagus and aphonic bark), with or without apparent neuromuscular weakness. It is a spontaneous model of human CMT4B2.
Inheritance patternAutosomal recessive. Confirmed affected dogs are homozygous for the SBF2 c.2363+1G>T variant; pedigree analysis and cosegregation support this mode of inheritance.
Gene / MutationSBF2/MTMR13 (CFA21), exon 19: c.2363+1G>T (splice donor; CanFam3.1 chr21:g.33080022C>A), which induces a cryptic splice site with a 40-bp deletion from the 3' end of exon 19 and a predicted truncation p.(Gly775Valfs*6).
PenetranceHigh in homozygotes; clinical expressivity is variable (some dogs with megaesophagus and aphonic bark without evident weakness). Heterozygotes are carriers without signs.
Codervao
Turnaround time7 days
Price42,10 €

Incidence

Breed in which it has been described: Miniature Schnauzer. In a screen of 802 genomes from 162 breeds/mixed breeds/wolves, 2 heterozygous Miniature Schnauzers and 1 mutant homozygote were found; the rest were clear. There are no reliable frequency figures in the general breeding population (limited data).

Breeder management

- In the event of a confirmed case, do not repeat the parental mating (both parents are obligate carriers)
- Genotype breeding animals with the SBF2-specific molecular test before breeding
- Do not breed two carriers together: 25 % risk of affected homozygotes
- A carrier may be bred to a clear animal; the offspring intended for breeding must be tested
- Withdraw affected animals from breeding
- Inform the buyer of the genetic status and monitor aspiration pneumonia in affected dogs

Specialist notes

Differential diagnosis with other polyneuropathies (diabetic neuropathy, senile neuropathy), myopathies and radiculopathies. Electromyography and nerve conduction velocity guide the diagnosis; nerve biopsy confirms demyelination with folded myelin. The clinical key in this breed is the combination of regurgitation with megaesophagus and aphonic bark. The molecular basis is well characterized and a direct genetic test is available.

References

1. Granger N, Luján Feliu-Pascual A, Spicer C, et al. Charcot-Marie-Tooth type 4B2 demyelinating neuropathy in miniature Schnauzer dogs caused by a novel splicing SBF2 (MTMR13) genetic variant: a new spontaneous clinical model. PeerJ. 2019;7:e7983. PMID: 31772832
2. Farré Mariné A, Granger N, Bertolani C, et al. Long-term outcome of Miniature Schnauzers with genetically confirmed demyelinating polyneuropathy: 12 cases. J Vet Intern Med. 2020;34(5):2005-2011. PMID: 32738000
3. Vanhaesebrouck AE, et al. Demyelinating polyneuropathy with focally folded myelin sheaths in a family of Miniature Schnauzer dogs. J Neurol Sci. 2008;275(1-2):100-105. PMID: 18809183

Add to cart

← Back to the search