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Neuronal ceroid lipofuscinosis 8 (NCL8) of the English Setter

Neurological · Dog

Neuronal ceroid lipofuscinosis of the English Setter caused by a missense mutation in CLN8. It accumulates autofluorescent material in neurons and produces progressive neurodegeneration with onset from juvenile to young adult. It is inherited in an autosomal recessive manner.
Inheritance patternAutosomal recessive
Gene / MutationCLN8 (chromosome 37, CanFam3.1): g.30874779T>C, NM_001012343.1:c.491T>C, p.(Leu164Pro), in the English Setter (OMIA001506; Katz et al. 2005, PMID:15629147). Other CLN8 variants cause NCL in the Saluki, Alpenländische Dachsbracke (complete deletion of the gene) and Australian Shepherd/GSP/Huntaway/NZ Heading dog.
PenetranceComplete penetrance in homozygotes, with onset around one or two years of age. Heterozygotes are asymptomatic.
Codexbsm
Turnaround time7 days
Price26,73 €

Incidence

Breed originally described: English Setter. In OMIA only the c.491T>C variant is listed for the English Setter; there is no verifiable source (PubMed or OMIA) showing that the Northern inuit or the Tamaskan carry this variant. Limited data on population frequencies.

Breeder management

- Genotype English Setter breeding animals before mating.\n- Do not cross two carriers: 25 % risk of affected homozygotes.\n- Progressively replace carrier lines while preserving genetic diversity.\n- A carrier can be crossed with a clear animal; the offspring intended for breeding must be tested.\n- Exclude affected animals from breeding.

Specialist notes

Differential diagnosis with other canine NCLs. The English Setter has been a model for gene therapy and for inhibitor trials for human NCL.

References

1. Katz ML, Khan S, Awano T, Shahid SA, Siakotos AN, Johnson GS. A mutation in the CLN8 gene in English Setter dogs with neuronal ceroid-lipofuscinosis. Biochem Biophys Res Commun 2005. PMID:15629147.
2. Katz ML, Rustad E, Robinson GO, et al. Canine neuronal ceroid lipofuscinoses: Promising models for preclinical testing of therapeutic interventions. Neurobiol Dis 2017. PMID:28860089.
3. Guo J, Johnson GS, Brown HA, et al. A CLN8 nonsense mutation in the whole genome sequence of a mixed breed dog with neuronal ceroid lipofuscinosis and Australian Shepherd ancestry. Mol Genet Metab 2014. PMID:24953404.
4. Hirz M, Drögemüller M, Schänzer A, et al. Neuronal ceroid lipofuscinosis (NCL) is caused by the entire deletion of CLN8 in the Alpenländische Dachsbracke dog. Mol Genet Metab 2017. PMID:28024876.

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