Test Detail

Osteogenesis imperfecta (brittle bone disease) Golden Retriever

Musculoesquelético · Dog

Osteogenesis imperfecta (OI), known as brittle bone disease, is an inherited connective tissue disorder that causes bone fragility and fractures from minimal or spontaneous trauma. In the Golden Retriever, a form associated with a mutation in COL1A1 (type I collagen alpha-1) has been described, one of the chains of type I collagen, the most abundant structural protein of bone, tendons and skin. The molecular test detects the known variant.
Inheritance patternAutosomal dominant (OMIA:002126-9615).
Gene / MutationCOL1A1 (canine chromosome 9). Published variant: c.1145G>C p.(Gly382Ala) (previous nomenclature: c.1276G>C p.(Gly208Ala)); missense substitution that breaks the Gly-X-Y pattern of the triple helix (Campbell 2000; OMIA variant 959).
PenetranceLimited data: there are no quantitative estimates of penetrance in the breed. Inheritance is dominant, so heterozygotes may be affected (OMIA:002126-9615).
Codetagl
Turnaround time7 days
Price36,05 €

Incidence

Documented sporadically in the Golden Retriever; there are no verifiable population frequency estimates (limited data). Most of the described cases are diagnosed in the first months of life.

Breeder management

- Do not breed affected animals.
- Since inheritance is dominant, review the pedigree and test relatives before using them as breeding animals.
- Avoid matings between lines with a history of OI.
- Keep a record of genetic results in the pedigree.

Specialist notes

The diagnosis is suspected by recurrent fractures, deformities and family history, and is confirmed with the genetic test. There is no cure: management is supportive (analgesia, treatment of fractures, physiotherapy and pain control). The prognosis depends on severity; mild cases can have a relatively normal life and severe cases require intensive management. It is important not to confuse the COL1A1 form with OI due to COL1A2 or SERPINH1, which require specific tests.

References

1. Campbell BG, Wootton JA, Krook L, DeMarco JA, Minor RR. Clinical signs and diagnosis of osteogenesis imperfecta in three dogs. J Am Vet Med Assoc. 1997;211(2):183-187. PMID: 9227748.
2. Campbell BG, Wootton JA, MacLeod JN, Minor RR. Sequence of normal canine COL1A1 cDNA and identification of a heterozygous alpha1(I) collagen Gly208Ala mutation in a severe case of canine osteogenesis imperfecta. Arch Biochem Biophys. 2000;384(1):37-46. PMID: 11147834.
3. Campbell BG, Wootton JA, Macleod JN, Minor RR. Canine COL1A2 mutation resulting in C-terminal truncation of pro-alpha2(I) and severe osteogenesis imperfecta. J Bone Miner Res. 2001;16(6):1147-1153. PMID: 11393792.
4. Drögemüller C, Becker D, Brunner A, et al. A missense mutation in the SERPINH1 gene in Dachshunds with osteogenesis imperfecta. PLoS Genet. 2009;5(7):e1000579. PMID: 19629171.
5. OMIA:002126-9615. Osteogenesis imperfecta, type III, COL1A1-related in Canis lupus familiaris. https://omia.org/OMIA002126/9615/

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