Test Detail

Feline erythrocytic pyruvate kinase deficiency (PK)

Hematológico · Cat

Erythrocytic pyruvate kinase (PK) deficiency is an inherited haemolytic anaemia caused by variants in the PKLR gene, which encodes the glycolytic enzyme pyruvate kinase. The defective enzyme shortens the lifespan of the erythrocyte and produces haemolytic anaemia of highly variable onset and severity, from mild compensated forms to severe anaemia with lethargy, jaundice and splenomegaly.
Inheritance patternAutosomal recessive.
Gene / MutationPKLR c.693+304G>A (intronic transition, 304 bp 3' of the splice donor site of exon 5); it produces a 13 bp deletion at the 3' end of exon 5 in the mRNA and a stop codon at amino acid 248 (OMIA:000844-9685).
PenetranceThe classic concept of penetrance does not apply: homozygotes present disease with great variability in onset and severity (Grahn et al. 2012, PMID 23110753); heterozygotes are clinically normal carriers. Precise penetrance figures: limited data.
Codejzlz
Turnaround time7 days
Price34,22 €

Incidence

Grahn et al. (2012, PMID 23110753) genotyped 14,179 cats from 38 breeds: the variant appears in 15 groups, with a mean frequency of 9.35 % (range 0.078 % in the Exotic Shorthair to 12.97 % in the Bengal). They recommend the test in the Bengal, Egyptian Mau, LaPerm, Maine Coon, Norwegian Forest Cat, Savannah, Siberian, Singapura, Abyssinian and Somali, as well as random-bred and domestic shorthair cats.

Breeder management

- PK deficiency is autosomal recessive: only homozygotes are affected; heterozygotes are healthy carriers.
- Do not mate two carriers together (25 % risk of affected offspring).
- A carrier may be mated with a clear animal, prioritising clear offspring to reduce the allele.
- In the case of haemolytic anaemia of unclear cause, include PK in the differential diagnosis and request the genetic test.
- Record the results in the pedigree; the test is recommended in the breeds with the highest documented frequency.

Specialist notes

The differential diagnosis of feline haemolytic anaemia includes haemoglobinopathies, haemotropic mycoplasmosis (Mycoplasma haemofelis), intoxications (onion, paracetamol, zinc), immune haemolysis and other enzymopathies. PK should be suspected in chronic haemolytic anaemia with reticulocytosis in predisposed breeds. There is no specific treatment; management is supportive and, in severe cases, transfusional.

References

1. Kohn B, Fumi C. 2008, curso clínico de la deficiencia de piruvato quinasa en gatos Abisinio y Somalí (PMID 18077199)
2. Grahn RA et al. 2012, mutación de la deficiencia de piruvato quinasa eritrocítica identificada en múltiples razas de gatos domésticos (PMID 23110753)
3. OMIA:000844 Deficiencia de piruvato quinasa felina

Add to cart

← Back to the search